Optical spectrometers (often simply called "spectrometers"), in particular, show the intensity of light as a function of wavelength or of frequency. The different wavelengths of light are separated by refraction in a prism or by diffraction by a diffraction grating. Spectrophotometer techniques are mostly used to measure the concentration of solutes in solution by measuring the amount of the light that is absorbed by the solution in a cuvette placed in the. Examples of spectrometers are devices that separate particles, atoms, and molecules by their mass, momentum, or energy. Spectrum of light emitted by a deuterium lamp in the UV, visible and near infrared part of the. Spectrophotometry and different types of spectroscopy are the technique that involved in identifying and quantifying the amount of a known substance in an unknown medium. Spectroscopy is the most convenient method for analysis of unknown samples both qualitatively and quantitatively with a good. ues. When light passes through a sample, the molecules in the sample absorb some of it, and the rest passes through. A digital spectrophotometer is.
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